| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60531SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22217SS | |||
| Azide and BSA Free | NBP2-80825 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1869SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | NBP1-22439AF350 | |||
| Alexa Fluor 532 | NBP1-22439AF532 | |||
| Alexa Fluor 594 | NBP1-22439AF594 | |||
| Alexa Fluor 750 | NBP1-22439AF750 | |||
| DyLight 594 | NBP1-22439DL594 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61895-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05771SS | |||
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another. Synapse-associated protein Synapsin1 is found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-54745-3) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0013.jpg "Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another. Synapse-associated protein Synapsin1 is found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-54745-3) licensed under a CC-BY license.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT and slow ESCs differentiated to neurons. Immunofluorescence staining for neuronal markers Map2, and Syn1 in neurons cultured on poly-ornithine-/laminin-coated plates. Expression of neuronal markers in neurons cultured on poly-ornithine-/laminin-coated plates from RNA-seq analysis, n = 3. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/embj.2018101244) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0014.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - Western blot analysis showing induced expression of neuronal functional marker Synapsin I at protein levels in MNC (meningial neuronal construct). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30443545/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synapsin I Antibody-Western Blot-NB300-104-img0015.jpg)
![Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104] - Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval at pH 6. Image from verified customer review.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunohistochemistry-Paraffin-NB300-104-img0012.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Western-Blot-NB300-104-img0007.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Western-Blot-NB300-104-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of Synapsin 1 (green) and MAP (red) proteins in a neuronal cell.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT & slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, & NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated plates.Expression of neuronal markers in neurons cultured on poly‐ornithine‐/laminin‐coated plates from RNA‐seq analysis, n = 3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30988016), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-310202415394528.jpg)
![Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another (a) through different types of synapses-like interactions, including dendrodendritic-like, axoaxonic-like & axodendritic-like synapses (b). (c) Synapse-associated proteins Cx43, Synaptophysin & Synapsin1 are found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 μm. AA, axoaxonic-like synapse; AD, axodendritic-like synapse; DD, dendrodendritic-like synapse; LM, light microscopy; SEM, scanning electron microscopy. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31792338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-310202415371980.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - Synaptic localization of TA at the PC–PF synaptic contacts.Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein (PSD95) & Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) & TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C–E) The high magnification of the inset in B is reproduced in different merge images & by the relative split channels. C) Merge image of Cb & V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D) Merge image of TA & Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E) Merge image of TA & V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) & TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23840813), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-31020241621237.jpg)
![Western Blot: Synapsin I Antibody [NB300-104] - Tau oligomers induce synaptic dysfunction. (A) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, synapsin-1, & septin-11 were measured by band quantification & normalized with the levels of tubulin. PBSo indicates representative bands of hippocampal area injected with PBS in mice also injected with tau oligomers, PBSf indicates PBS injection in mice also injected with tau fibrils, & PBSm indicates PBS injection in mice also injected with tau monomers. (B) Synaptophysin levels were significantly lower in the hemispheres injected with tau oligomers in comparison with the ones injected with fibrils, monomers, or PBS. (C) No significant differences in the levels of synapsin-1 were observed. (D) Only the hemisphere injected with tau oligomers presents a decrease in the level of septin-11. Data are represented as the mean ± SE. *p < 0.01, n = 6 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21645391), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-104_rabbit-polyclonal-synapsin-i-antibody-31020241620516.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunohistochemistry-Paraffin-NB110-60531-img0011.jpg "Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.")
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0013.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0014.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE (WUE-4) [NB110-60531] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunocytochemistry-Immunofluorescence-NB110-60531-img0010.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex beads. The EXOs were isolated from fasting PFP or PLT concentrate by differential UC and gravity size filtration (gray histograms: blocked beads incubated with antibody, empty histograms: EXO sample). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep24316), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0020.jpg)
![Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Intracellular-NB110-60531-img0012.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0015.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0016.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0017.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0018.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0019.jpg)
![Knockout Validated: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human LAMP-2/CD107b (H4B4) Monoclonal Antibody (Catalog # NBP2-22217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LAMP-2/CD107b at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Knockout-Validated-NBP2-22217-img0016.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human LAMP-2/CD107b (H4B4) Monoclonal Antibody (Catalog # NBP2-22217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LAMP-2/CD107b at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Immunofluorescence: [Alexa Fluor® 647] [NBP2-22217AF647] - T98G glioblastoma cells grown on #1.5 coverglass stained with a conjugated LAMP-2/CD107b (H4B4)AF647 antibody, counterstained with DAPI. Cathepsin D (green) staining also visible. This image was submitted via customer Review. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Immunocytochemistry-Immunofluorescence-NBP2-22217-img0015.jpg)
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human liver, and 9) Human placenta.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Western-Blot-NBP2-22217-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Immunohistochemistry-Paraffin-NBP2-22217-img0001.jpg)
![Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Flow-Cytometry-NBP2-22217-img0017.jpg)
![Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Simple-Western-NBP2-22217-img0005.jpg)
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Localization of TMEM135 to the mitochondria. The mitochondrial fraction isolated from the WT mouse brain show TMEM135 signals by immunoblotting. Following proteins were used as organelle markers: MFN2-mitochondria; LAMP2-lysosome; Lamin B1- nucleus; PDI- endoplasmic reticulum (ER). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19264), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Western-Blot-NBP2-22217-img0019.jpg)
![Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. Western blot analysis expression level of Cathepsin B (*P < 0.05) in total protein extract from RVA of I/R treated vs. control group. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01034/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Western-Blot-NBP2-22217-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LAMP-2/CD107b [NBP2-22217] at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Immunocytochemistry-Immunofluorescence-NBP2-22217-img0008.jpg)
![Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-Flow-Cytometry-NBP2-22217-img0004.jpg)
![ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-H4B4-ELISA-NBP2-22217-img0014.jpg)
![Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Intracellular-NB100-1869-img0011.jpg "Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).")
![Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-NB100-1869-img0005.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Western-Blot-NB100-1869-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at 1:250 in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0025.jpg)
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 on mouse brain.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NB100-1869-img0006.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - An intracellular stain was performed on rat FR cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody---BSA-Free-Flow-Cytometry-NB100-1869-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0024.jpg)
![Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/mL) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) isotype control (blue).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Intracellular-NB100-1869-img0012.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0019.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0020.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0021.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Caco-2 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0022.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Neuro2a cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0023.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415304223.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415382418.jpg)
![Simple Western: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-31020241553387.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Depletion of microglia promotes astrocyte responses following MPTP treatment. C57BL/6 mice received PLX3397 or vehicle for 21 d prior to saline or MPTP administration (intraperitoneal injection). Mice continued to receive PLX3397 or vehicle until the experiments ended. At d 7 after saline or MPTP injection, single cell suspensions were prepared from substantia nigra & striatal tissues. A) Flow cytometry plots show the gating strategy for astrocytes (GLAST+) & their expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21. B, C) Bar graphs show the effects of microglial depletion on the expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21 in astrocytes at d 7 after MPTP treatment. All data are presented as means ± sem; n = 9 mice/group. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29401614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415525277.jpg)
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Analysis of extracellular levels of GABA & Glutamate & membrane expression of their transporters. Extracellular levels of (A,C) GABA & glutamate were analyzed by microdialysis at 4 weeks. Membrane expression of (B) glutamate transporter GLAST & (D,E) GABA transporters GAT1 & GAT3 were analyzed using BS3 cross-linker. Values are expressed as percentage of control rats & are mean ± SEM of 12 rats per group. One-way ANOVA with Tukey’s test for GAT-1 (F(3,27) = 4.41, p < 0.05), GAT-3 (F(3,41) = 3.27, p < 0.05) & glutamate (F(3,36) = 3.00, p < 0.05) & Welch’s ANOVA with Dunnett’s test for GABA (W(3,19) = 3.67, p < 0.05) & GLAST (W(3,13) = 12.07, p < 0.001) were performed to compare all groups. Values significantly different from control rats are indicated by asterisks, from CCl4 rats by a, & from C-RIF rats by b. * p < 0.05, a p < 0.05, b p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415523910.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0012.jpg "Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Normal deparaffinized Human placenta tissue tissues stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0015.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0016.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 25ug of NRK cell lysates using anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0017.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Validation of subcellular fractionation and immunocytochemistry. RLC proteins are present at ER microsomes. Lysates from 7 DIV cortical neurons were subjected to differential centrifugation to isolate nuclear pellet (P1), postnuclear supernatant (PNS), mitochondrial pellet (P2), and ER microsomal pellet (P3), as depicted in the graphic on the right. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embr.201744853) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0019.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 10 ug of HeLa (left panel), NIH/3T3 (middle panel) and NRK (right panel) whole cell lysates using anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b (green) in NIH/3T3 cells.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0013.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human tonsils were stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0014.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Exposure of rat primary microglial cells (rPMs) to combined antiretroviral therapy (cART) cocktail resulted in impaired lysosomal function. rPMs were seeded into six-well plates & treated with cART (5 µM each of tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), & dolutegravir (DTG)) for the indicated time periods. (A,B) Exposure of microglia to cART resulted in a significant decrease in expression of lysosomal-associated membrane protein 2 (LAMP2) at 6 to 24 h post-treatment. (C) Representative bar graph showing cART-mediated significantly increased lysosomal membrane permeabilization (LMP) (24 h). (D,E) Microglia exposed to cART demonstrated a significant decrease in levels of mature cathepsin D (mCTSD) at 24 h post-treatment. (F) Representative bar graph showing exposure of cART significantly reduced the CTSD activity in rPMs (24 h). (G) Representative bar graph showing cART-mediated increased lysosomal pH in rPMs. (H,I) Acridine orange staining showing increased green color & reduced red color in cART-treated rPMs. H2O2 was used as a positive control for lysosome damage. Data is from three independent experiments. Actin beta (ACTB) served as a protein loading control for western blots. Data are expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; N.S., non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-591_rabbit-polyclonal-lamp-2-cd107b-antibody-310202416175213.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - HSPA overexpression abrogated cART-mediated impairment of lysosomal function. Control rPMs & heat shock protein family A (HSPA) overexpressing rPMs were seeded into six-well plates subjected to various treatments for 24 h. Protein homogenates were prepared for the detection of the indicated molecules. (A,B) Representative western blots showing overexpressing HSPA in rPMs reversed cART-mediated downregulation of LAMP2 & mCTSD expression levels. (C) Representative bar graph showing overexpression of HSPA in rPMs protected lysosomal pH. (D,E) Representative bar graphs showing HSPA protected LMP (D), & CTSD activity (E) in cART-treated rPMs. For all western blots, ACTB served as a protein loading control. Data is from three independent experiments & is expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; #, p < 0.05 vs. cART. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-591_rabbit-polyclonal-lamp-2-cd107b-antibody-31020241618933.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Western-Blot-NBP1-22439-img0015.jpg "Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0018.jpg)
![Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (NBP1-22439) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunohistochemistry-NBP1-22439-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0019.jpg)
![Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunohistochemistry-NBP1-22439-img0016.jpg)
![Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Knockdown-Validated-NBP1-49540-img0007.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP1-49540-img0004.jpg)
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-NBP1-49540-img0005.jpg)
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Simple Western lane view shows a specific band for SIRT1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP1-49540-img0006.jpg)
![Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Western-Blot-NBP2-61895-img0009.jpg "Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Immunocytochemistry-Immunofluorescence-NBP2-61895-img0006.jpg)
![Immunohistochemistry: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of paraffin-embedded testis tissues using SYN1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Immunohistochemistry-NBP2-61895-img0007.jpg)
![Flow Cytometry: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of Hela cells using SYN1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Flow-Cytometry-NBP2-61895-img0002.jpg)
![Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Western-Blot-NBP2-61895-img0008.jpg)
![Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mouse mAb against SK-N-SH (1), NIH/3T3 (2), U251 (3), C6 (4), A549 (5), MCF-7 (6), and COS7 (7) cell lysate.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Western-Blot-NBP2-61895-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of GC-7901 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Immunocytochemistry-Immunofluorescence-NBP2-61895-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of GC-7901 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Immunocytochemistry-Immunofluorescence-NBP2-61895-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-Immunocytochemistry-Immunofluorescence-NBP2-61895-img0005.jpg)
![ELISA: Synapsin I Antibody (7H10G6) [NBP2-61895] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Synapsin-I-Antibody-7H10G6-ELISA-NBP2-61895-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40773] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40773-img0006.jpg "Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40773] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
![Immunohistochemistry: DISC1 Antibody [NB110-40773] - Staining of DISC1 in human brain neurons and neuropil at 2.5ug/ml. 40X.](http://images.novusbio.com/fullsize/DISC1-Antibody-Immunohistochemistry-NB110-40773-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05771-img0004.jpg "Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- EAAT1/GLAST-1/SLC1A3 Antibody NBP3-05771 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of SLC1A3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong cytoplasmic and membrane reactivity were observed.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NBP3-05771-img0001.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on Hek293 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NBP3-05771-img0003.jpg)
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NBP3-05771] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NBP3-05771 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NBP3-05771-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - Staining of CACNA1C in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76914-img0008.jpg "Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - Staining of CACNA1C in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0006.jpg)
![Flow Cytometry: Cav1.2 Antibody [NBP2-76914] - Flow cytometric analysis of SKOV-3 cells with CACNA1C antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Flow-Cytometry-NBP2-76914-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - ICC staining CACNA1C in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76914-img0002.jpg)
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0003.jpg)
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0004.jpg)
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0005.jpg)
![Dot Blot: Cav1.2 Antibody [NBP2-76914] - Dot blot analysis of anti-CACNA1C on PVDF. 1ug, 2ug and 4ug of immunization peptides were given in this test. Anti-CACNA1C antibody was diluted with 1/500.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Dot-Blot-NBP2-76914-img0001.jpg)